. Itekhnoloji yetekhnoloji ephezulu iphuhliswe ngokusekwe kuhlalutyo kunye nokuhlelwa kweeseli enye. Inokulinganisa ngokukhawuleza kwaye ihlule ubungakanani, isakhiwo sangaphakathi, iDNA, i-RNA, iiproteni, iiproteni kunye nezinye iiseli zomzimba okanye zekhemikhali zeeseli, kwaye zinokusekelwa ekuqokeleleni kolu hlaziyo.
Ukuhamba kwe-cytometer ikakhulu kubandakanya la macandelo mahlanu alandelayo:
I-1 yegumbi lokuhamba kunye nenkqubo yefladi
I-2 i-laser yokukhanya kunye nenkqubo ye-beam
I-3 inkqubo yamathuba
Ii-Elektroniki, ukugcina, ukubonisa kunye nenkqubo yokuhlaziya
Inkqubo yokuhlela kwiseli ezi-5
Phakathi kwawo, i-serction kwi-laser ye-laser kunye nenkqubo yokwakha i-Beam yeyona milinganiselo iphambili ye-fluoresces kwi-cytometry ye-cytometry. Ubunzulu bendlela yokukhanya kunye nexesha lokuvezwa luhambelana nobunzulu bophawu lwe-fluorescence. I-Laser ngumthombo wokukhanya onobunaba onokuthi anikezele ngelinye i-waveventth enye, ubukhulu obuphezulu, kunye nokukhanya okuphezulu. Luhlobo olufanelekileyo lomthombo wokuqulala ukufikelela kwezi mfuno.
Kukho iilensi ezimbini ze-cylindrical phakathi komthombo we-laser kunye negumbi lokuhamba. Ezi lelo zigxile kwi-laser ye-laser ngecandelo elijikelezileyo elikhutshelwe umthombo we-laser kwi-el proptical beam ngecandelo elincinci (22 μ 66 μ 6] μm). Amandla ase-laser kule bhaso le-elliptical lisasazwa ngokokusasazwa okuqhelekileyo, ukuqinisekisa ukuba ukhanyiso lukhulu lweeseli ezidlula kwindawo yokufumana indawo. Kwelinye icala, inkqubo yamatshecaphu iqulethe iiseti ezininzi zeelensi, kunye neefiltha, ezinokwahlulwa zangamaqela amabini: inyuka igumbi lokuhlambela kwigumbi lokuhamba.
Inkqubo ye-optical phambi kwegumbi lokuhamba line-lens kunye nepikhole. Umsebenzi ophambili welensi kunye nepinki (ihlala i-lenses ezimbini kunye ne-pinhole) kukugxila kwi-laser ye-laser ngecandelo elijikelezwe ngumthombo we-leser kwicandelo elincinci le-elliptical. Oku kusasaza amandla amanqam ngokokusasazwa okuqhelekileyo, ukuqinisekisa ukuba ukhanyiselwa okungakumbi kwiiseli kwindawo yokufumana i-laser kunye nokunciphisa ukuphazanyiswa kokukhanya okukhohlakeleyo.
Zintathu iintlobo eziphambili zefilitha:
1: Isihluzi eside sokudlula (i-LPF) - ivumela kuphela ukukhanya kunye namazandakali ngaphezulu kwexabiso elithile lokudlula.
2: Isihluzi se-DLAR-DLALA (SPF) -ivumela ukukhanya kunye ne-lavelengths ngaphantsi kwexabiso elithile lokudlula.
I-3: Isifiso se-Bandet
Indibaniselwano eyahlukeneyo yefilitha inokutsala umnxeba i-Futoresces imiqondiso eyahlukeneyo ye-tubes nganye ye-tubes (PMTS). Umzekelo, iifilitha zokuchonga i-fluorescence (fitc) phambi kwe-PMT i-LPF550 ne-BPF525. Iifilitha ezisetyenziselwa ukufumana i-orange-red fluorescence (i-PE) phambi kwe-PMT yi-LPF200 ne-BPF575. Iifilitha zokuchonga i-fluorescence (i-CY5) phambi kwe-PMT yi-LPF650 ne-BPF675.
Ukuhamba kwe-cytometry kusetyenziselwa ukuhlelwa kweseli. Ngokuqhubela phambili kwetekhnoloji yekhompyuter, ukuphuculwa kwe-Immunology kunye nokuyilwa kwetekhnoloji ye-anticlon, izicelo zayo kwi-biology, amayeza, ikhemisi kunye namanye amasimi ayanda ngakumbi. Ezi zicelo zibandakanya uhlalutyo lweeseli zeseli, i-apoptosis yeseli, ukuchwetheza iseli, ukuxilongwa kwe-tumas, uhlalutyo lwe-sumor, njl njl.
I-Post Time: Sep-21 ukuya kwi-2023
